人Th1/Th2/Th17抗体阵列-膜(34 targets) (ab169809)
概述
-
产品名称
人Th1/Th2/Th17抗体阵列-膜(34 targets)
参阅全部 Cytokine Antibody Array 抗体芯片 -
样品类型
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Whole Blood, Tissue Extracts, Cell Lysate, Cell culture media -
检测类型
Semi-quantitative -
种属反应性
与反应: Human -
产品概述
ab169809 is for the simultaneous detection of 34 Human Th1, Th2, and Th17 cytokines. Suitable for all liquid sample types.
Targets: CD30, CD40 Ligand, CD40, G-CSF, GITR, GM-CSF, IFN-gamma, IL-1 beta, IL-1 sRI, IL-1 sRII, IL-2, IL-4, IL-5, IL-6, IL-6 R, IL-10, IL-12 p40, IL-12 p70, IL-13, IL-17, IL-17F, IL-17 R, IL-21, IL-21R, IL-22, IL-23 (p19), IL-28A, MIP-3 alpha, spg130, TGF beta 1, TGF beta 3, TNF alpha, TNF beta, TRANCE.
Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.
Learn more about membrane antibody arrays
-
说明
A table listing all of our human membrane antibody cytokine arrays and other arrays and the analytes they measure is available here.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
经测试应用
适用于: Multiplex Protein Detectionmore details
性能
-
存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 4 Membranes 1 x 8 Membranes 1,000X HRP-Conjugated Streptavidin 1 x 50µl 1 x 50µl 1X Blocking Buffer 1 x 25ml 2 x 25ml 2000X Biotin-Conjugated Anti-Cytokines 1 x 2 vials 1 x 4 vials 20X Wash Buffer I 1 x 10ml 1 x 20ml 20X Wash Buffer II 1 x 10ml 1 x 20ml 2X Cell Lysis Buffer 1 x 10ml 1 x 16ml 8-Well Incubation Tray (with Lid) 1 unit 1 unit Angiogenesis Antibody Array Membranes 4 units 8 units Detection Buffer C 1 x 1.5ml 1 x 2.5ml Detection Buffer D 1 x 1.5ml 1 x 2.5ml -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab169809于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Multiplex Protein Detection |
Use at an assay dependent concentration.
|
说明 |
---|
Multiplex Protein Detection
Use at an assay dependent concentration. |
图片
-
Human serum and plasma (EDTA) from a pooled donor (n=50) sample was diluted to 25% and assayed using ab169809. Mean pixel density was quantified using CCD camera software analysis.
-
Human peripheral blood cells (1x106 cells/mL) were cultured in RPMI media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 mg/mL streptomycin sulfate. Cells were cultured unstimulated or stimulated with 10 mg/mL PHA. Conditioned media was harvested after 48 hours, aliquoted and assayed using ab169809. Mean pixel density was quantified using CCD camera software analysis.
-
Typical results obtained with Abcam Membrane Antibody Arrays. Image above presents typical images obtained with Abcam Human Cytokine Membrane Antibody Array. These membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at RT for 1 min.
数据表及文件
-
SDS download
-
Datasheet download
文献 (2)
ab169809 被引用在 2 文献中.
- Machcińska M et al. Reduced Expression of PD-1 in Circulating CD4+ and CD8+ Tregs Is an Early Feature of RRMS. Int J Mol Sci 23:N/A (2022). PubMed: 35328606
- Gauthier BR et al. Human Omental Mesothelial Cells Impart an Immunomodulatory Landscape Impeding B- and T-Cell Activation. Int J Mol Sci 23:N/A (2022). PubMed: 35682603