Anti-Heme Oxygenase 1抗体(ab13243)
Key features and details
- Rabbit polyclonal to Heme Oxygenase 1
- Suitable for: WB, ICC/IF, Sandwich ELISA, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Heme Oxygenase 1抗体
参阅全部 Heme Oxygenase 1 一抗 -
描述
兔多克隆抗体to Heme Oxygenase 1 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IF, Sandwich ELISA, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant rat HO-1 (Hsp32) lacking the membrane spanning region
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
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sELISA pair antibody
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab13243于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (3) |
1/2000. Detects a band of approximately 32 kDa (predicted molecular weight: 34.6 kDa).
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ICC/IF |
Use a concentration of 1 - 5 µg/ml.
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Sandwich ELISA |
Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [HO-1-1] to Heme Oxygenase 1 (ab13248).
For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [HO-1-1] to Heme Oxygenase 1 (ab13248) as Capture. |
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IHC-P | (3) |
Use a concentration of 1 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
1/2000. Detects a band of approximately 32 kDa (predicted molecular weight: 34.6 kDa). |
ICC/IF
Use a concentration of 1 - 5 µg/ml. |
Sandwich ELISA
Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [HO-1-1] to Heme Oxygenase 1 (ab13248). For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [HO-1-1] to Heme Oxygenase 1 (ab13248) as Capture. |
IHC-P
Use a concentration of 1 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. -
序列相似性
Belongs to the heme oxygenase family. -
细胞定位
Microsome. Endoplasmic reticulum. - Information by UniProt
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数据库链接
- Entrez Gene: 3162 Human
- Entrez Gene: 15368 Mouse
- Entrez Gene: 24451 Rat
- Omim: 141250 Human
- SwissProt: P09601 Human
- SwissProt: P14901 Mouse
- SwissProt: P06762 Rat
- Unigene: 517581 Human
see all -
别名
- 32 kD antibody
- bK286B10 antibody
- D8Wsu38e antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody (ab13243)
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All lanes : Anti-Heme Oxygenase 1 antibody (ab13243) at 1/1000 dilution
Lane 1 : Hek293
Lane 2 : HL60
Lane 3 : HeLa
Lane 4 : A549
Lane 5 : Hu spleen
Lane 6 : Ms spleen
Lane 7 : Rt spleen
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye® 800CW Goat anti Rabbit
Predicted band size: 34.6 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?Hek293 & HL60 presumed negative or very low expression.
Loading control GAPDH at 38kDa
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IHC image of Heme Oxygenase 1 staining in formalin fixed, paraffin embedded rat spleen normal tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab13243, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Anti-Heme Oxygenase 1 antibody (ab13243) (Rat microsomes)
Predicted band size: 34.6 kDa -
Alzheimer diseased section stained with ab13243.
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ICC/IF image of ab13243 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13243, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Standard Curve for Heme Oxygenase 1 (Analyte: Heme Oxygenase 1 protein (Tagged) (ab85243)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [HO-1-1] to Heme Oxygenase 1 (ab13248) at 5µg/ml and Detector Antibody Rabbit polyclonal to Heme Oxygenase 1 (ab13243) at 0.5µg/ml.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (235)
ab13243 被引用在 235 文献中.
- Guo Y et al. Hyperglycemia Induces Meibomian Gland Dysfunction. Invest Ophthalmol Vis Sci 63:30 (2022). PubMed: 35072689
- Matić S et al. Interdisciplinary Study of the Effects of Dipeptidyl-Peptidase III Cancer Mutations on the KEAP1-NRF2 Signaling Pathway. Int J Mol Sci 23:N/A (2022). PubMed: 35216111
- Wen H et al. Ruscogenins Improve CD-Like Enteritis by Inhibiting Apoptosis of Intestinal Epithelial Cells and Activating Nrf2/NQO1 Pathway. Oxid Med Cell Longev 2022:4877275 (2022). PubMed: 35308175
- Malakul W et al. Novel Coconut Vinegar Attenuates Hepatic and Vascular Oxidative Stress in Rats Fed a High-Cholesterol Diet. Front Nutr 9:835278 (2022). PubMed: 35356733
- Zhou X et al. Icaritin attenuates 6-OHDA-induced MN9D cell damage by inhibiting oxidative stress. PeerJ 10:e13256 (2022). PubMed: 35433120