Fast Bisulfite Conversion试剂盒(ab117127)
Key features and details
- Assay time: 30 min
概述
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产品名称
Fast Bisulfite Conversion试剂盒
参阅全部 DNA modification 试剂盒 -
检测时间
0h 30m -
产品概述
Fast DNA modification Kit (ab117127) allows DNA denaturation and bisulfite conversion to be processed at the same time so the complete procedure can be performed in only 30 minutes. Furthermore, it prevents more than 90% of DNA loss, completely converting unmethylated cytosine into uracil.
Traditional methods involve a separate denaturation step followed by a subsequent sodium bisulfite DNA conversion step - but with ab117127, DNA denaturation status is concurrently sustained throughout the entire bisulfite DNA conversion process. ab117127 is suitable for MS-PCR, real time MS-PCR, bisulfite sequencing, pyrosequencing and methylation microarray.
DNA methylation is a covalent modification of the cytosine ring at the 5' position of a CpG dinucleotide which leads to epigenetic inactivation of genes when found in 5'-CpG-3'dinucleotides within promoters or in the first exon of genes. It is well demonstrated that DNA methylation plays an important role in the regulation of gene expression, tumorigenesis, and other genetic and epigenetic diseases.
Most methods for DNA methylation detection require a prior bisulfite-based DNA modification. Bisulfite-based DNA modification is used to discrimate between cytosine and methylated cytosine, in which DNA is treated with bisulfite salt to convert cytosine residues to uracil in a single-stranded DNA, while methylated cytosine remains the same.
Not sure if this is the right kit for you? Check out our bisulfite modification guide for more information.
性能
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存放说明
Store at room temperature. Please refer to protocols. -
组件 50 tests Capture Solution 1 x 15ml Conversion Enhancer 5 vials Conversion Mix Solution 1 x 6ml Denaturation Enhancer 1 x 600µl Desulphonation Solution 1 x 60µl Elution Solution 1 x 1ml F-Collection Tube 1 pack F-Spin Column 1 pack -
研究领域
相关产品
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Related Products
图片
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Effective DNA Protection: Fully methylated human genomic DNA at various amounts (0.2 ng-200 ng) were converted using the ab117127. 1 µl of 20 µl eluate was used for real time qPCR and a pair of primers was used to amplify converted DNA. As little as 0.2 ng DNA is sufficient for bisulfite conversion using the ab117127. A: real time PCR; B: starting DNA amount-CT value curve.
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Complete Cytosine Conversion: 200 ng of genomic DNA isolated from 3 cancer cell lines was treated with ab117127. Next, the unconverted and converted DNA in each treated sample were determined using unconverted DNA-specific and converted DNA-specific primers (ß-actin, 110 bps), respectively. A: real time PCR; B: end-point PCR. The ab117127 treated DNA was completely converted, and no unconverted DNA in the treated samples was determined after 45 cycles.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab117127 被引用在 2 文献中.
- Yan B et al. Methyl-SNP-seq reveals dual readouts of methylome and variome at molecule resolution while enabling target enrichment. Genome Res 32:2079-2091 (2022). PubMed: 36332968
- Kint S et al. Evaluation of bisulfite kits for DNA methylation profiling in terms of DNA fragmentation and DNA recovery using digital PCR. PLoS One 13:e0199091 (2018). PubMed: 29902267