重组人NFkB Inducing Kinase NIK蛋白(ab105210)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 85% SDS-PAGE
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE
描述
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产品名称
重组人NFkB Inducing Kinase NIK蛋白
参阅全部 NFkB Inducing Kinase NIK 蛋白酶 -
生物活性
The Specific activity of ab105210 was determined to be 8 nmol/min/mg.
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纯度
> 85 % SDS-PAGE.
Purity was determined to be >85% by densitometry. Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Human -
预测分子量
108 kDa including tags -
氨基酸
325 to 947
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相关产品
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Substrate reagent
技术指标
Our Abpromise guarantee covers the use of ab105210 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Western blot
Functional Studies
SDS-PAGE
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形式
Liquid -
补充说明
ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.307% Glutathione, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- MAP3K14
- FTDCR1B
- HS
see all -
功能
Lymphotoxin beta-activated kinase which seems to be exclusively involved in the activation of NF-kappa-B and its transcriptional activity. Promotes proteolytic processing of NFKB2/P100, which leads to activation of NF-kappa-B via the non-canonical pathway. Could act in a receptor-selective manner. -
组织特异性
Weakly expressed in testis, small intestine, spleen, thymus, peripheral blood leukocytes, prostate, ovary and colon. -
序列相似性
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 1 protein kinase domain. -
翻译后修饰
Autophosphorylated. Phosphorylation at Thr-559 is required to activates its kinase activity and 'Lys-63'-linked polyubiquitination. Phosphorylated by CHUK/IKKA leading to MAP3K14 destabilization.
Ubiquitinated. Undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. 'Lys-48'-linked polyubiquitination leads to its degradation by the proteasome, while 'Lys-63'-linked polyubiquitination stabilizes and activates it. -
细胞定位
Cytoplasm. - Information by UniProt
图片
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The specific activity of NFkB Inducing Kinase NIK (ab105210) was determined to be 5.5 nmol/min/mg as per activity assay protocol and was equivalent to 7.1 nmol/min/mg as per radiometric assay
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SDS PAGE analysis of ab105210
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SDS PAGE analysis of ab105210
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The Specific activity of ab105210 was determined to be 8 nmol/min/mg.
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SDS-PAGE showing ab105210 at approximately 108kDa.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab105210 尚未被引用在任何文献中。