抗人Serum Albumin抗体[15C7] (ab10241)
Key features and details
- Mouse monoclonal [15C7] to Human Serum Albumin
- Suitable for: WB, Sandwich ELISA, Dot blot
- Reacts with: Human
- Isotype: IgG2b
概述
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产品名称
抗人Serum Albumin抗体[15C7]
参阅全部 Human Serum Albumin 一抗 -
描述
小鼠单克隆抗体[15C7] to人Serum Albumin -
宿主
Mouse -
特异性
No cross-reactivity with other human proteins tested. Does not react with bovine serum albumin, egg white albumin or human alpha fetoprotein.
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经测试应用
适用于: WB, Sandwich ELISA, Dot blotmore details -
种属反应性
与反应: Human
不与反应: Chicken, Cow -
免疫原
Full length native protein (purified) corresponding to Human Serum Albumin.
Database link: P02768 -
常规说明
Concentration varies from lot to lot and can be provided on request.
This product was changed from ascites to tissue culture supernatant on 17th October 2017 and product received after this date will be from tissue culture supernatant.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
15C7 -
骨髓瘤
Sp2/0 -
同种型
IgG2b -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
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sELISA pair antibody
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab10241于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (2) |
Use at an assay dependent concentration. Predicted molecular weight: 65 kDa.
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Sandwich ELISA |
Use a concentration of 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Human Serum Albumin (ab2406).
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Dot blot |
Use at an assay dependent concentration.
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说明 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 65 kDa. |
Sandwich ELISA
Use a concentration of 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Human Serum Albumin (ab2406). |
Dot blot
Use at an assay dependent concentration. |
靶标
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功能
Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. -
组织特异性
Plasma. -
疾病相关
Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population. -
序列相似性
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
翻译后修饰
Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
Glycated in diabetic patients.
Phosphorylation sites are present in the extracelllular medium.
Acetylated on Lys-223 by acetylsalicylic acid. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 213 Human
- Omim: 103600 Human
- SwissProt: P02768 Human
- Unigene: 418167 Human
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别名
- alb antibody
- ALBU_HUMAN antibody
- Albumin (32 AA) antibody
see all
图片
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Dot Blot - Anti-Human Serum Albumin antibody [15C7] (ab10241)
Lane 1: HSA 0.5 mg/ml
Lane 2: HSA 0.25 mg/ml
Lane 3: HSA 0.1 mg/ml
Lane 4: HSA 0.01 mg/ml
Lane 5: BSA 0.5 mg/ml
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Anti-Human Serum Albumin antibody [15C7] (ab10241) at 1/2000 dilution (in 5% milk for 1 hour) + Human blood serum at 2 µg
Secondary
anti-mouse IgG (H&L)
Developed using the ECL technique.
Predicted band size: 65 kDa
Exposure time: 5 seconds
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Detection of Human Serum Albumin by ab10241 in western blotting.
Lane 1 - human serum albumin, 3 µg.
Lane 2 - normal human serum, ~3 µg of human serum albumin.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (39)
ab10241 被引用在 39 文献中.
- Sastre-Oliva T et al. The Influence of Coronary Artery Disease in the Development of Aortic Stenosis and the Importance of the Albumin Redox State. Antioxidants (Basel) 11:N/A (2022). PubMed: 35204200
- Hou W et al. Serum Extracellular Vesicle Stratifin Is a Biomarker of Perineural Invasion in Patients With Colorectal Cancer and Predicts Worse Prognosis. Front Oncol 12:912584 (2022). PubMed: 35936690
- Bayarsaikhan D et al. A Study on the Protective Effect of sRAGE-MSCs in a Rodent Reperfusion Model of Myocardial Infarction. Int J Mol Sci 23:N/A (2022). PubMed: 36555270
- Taymour R et al. 3D bioprinting of hepatocytes: core-shell structured co-cultures with fibroblasts for enhanced functionality. Sci Rep 11:5130 (2021). PubMed: 33664366
- Dimov N et al. Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay. J Vis Exp N/A:N/A (2020). PubMed: 32150159