Key features and details
- Rabbit polyclonal to AANAT (phospho T29)
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
产品名称Anti-AANAT (phospho T29)抗体
参阅全部 AANAT 一抗
描述兔多克隆抗体to AANAT (phospho T29)
经测试应用适用于: WBmore details
预测可用于: Mouse, Chicken, Hamster, Human, Chimpanzee, Rhesus monkey
- Rat pineal gland whole protein extract.
This product was previously labelled as Serotonin N-acetyltransferase
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS
Concentration information loading...
纯度Immunogen affinity purified
纯化说明Total anti-N-terminal AA-NAT-specific IgG was affinity purified on resin-coupled non-phosphopeptide. Phospho-specific N-terminal IgG was subsequently isolated on resin-coupled phosphopeptide.
Primary antibody说明Pineal gland Serotonin N-acetyltransferase (Arylalkylamine N-acetyltransferase, AA-NAT) is an ~23 kDa protein which is the rate-limiting enzyme in melatonin synthesis. Melatonin serves as the hormonal signal of the daily light / dark cycle, or body clock. The nocturnal oscillation in enzyme activity ranges by 10- to 100-fold depending on species among vertebrates. Studies have shown that AA-NAT is regulated by cAMP at both the mRNA and protein levels. AA-NAT has putative protein kinase A (PKA) sites at Thr 29 and Ser 206. Phosphorylation of AA-NAT at these residues might play a role in protein inactivation via proteasome-dependent degradation.
Our Abpromise guarantee covers the use of ab3439 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
By Western blot, this antibody detects an ~23 kDa protein representing phospho AA-NAT in rat pineal gland whole protein extract.
功能Controls the night/day rhythm of melatonin production in the pineal gland. Catalyzes the N-acetylation of serotonin into N-acetylserotonin, the penultimate step in the synthesis of melatonin.
组织特异性Highly expressed in pineal gland and at lower levels in the retina. Weak expression in several brain regions and in the pituitary gland.
通路Aromatic compound metabolism; melatonin biosynthesis; melatonin from serotonin: step 1/2.
疾病相关Delayed sleep phase syndrome
序列相似性Belongs to the acetyltransferase family. AANAT subfamily.
Contains 1 N-acetyltransferase domain.
翻译后修饰cAMP-dependent phosphorylation on both N-terminal Thr-31 and C-terminal Ser-205 regulates AANAT activity by promoting interaction with 14-3-3 proteins.
- Information by UniProt
- AA-NAT antibody
- Aanat antibody
- Aralkylamine N-acetyltransferase antibody
ab3439 被引用在 2 文献中.
- Hou B et al. Homeostatic Plasticity Mediated by Rod-Cone Gap Junction Coupling in Retinal Degenerative Dystrophic RCS Rats. Front Cell Neurosci 11:98 (2017). IHC (PFA fixed) ; Rat . PubMed: 28473754
- Moran AW et al. Expression of Na+/glucose co-transporter 1 (SGLT1) is enhanced by supplementation of the diet of weaning piglets with artificial sweeteners. Br J Nutr 104:637-46 (2010). PubMed: 20338074