Anti-AANAT抗体(ab3506)

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Western blot - Anti-AANAT antibody (ab3506)

    Key features and details

    • Rabbit polyclonal to AANAT
    • Suitable for: WB
    • Reacts with: Rat
    • Isotype: IgG

    概述

    • 产品名称

      Anti-AANAT抗体
      参阅全部 AANAT 一抗

    • 描述

      兔多克隆抗体to AANAT

    • 宿主

      Rabbit

    • 经测试应用

      适用于: WBmore details

    • 种属反应性

      与反应: Rat
      预测可用于: Mouse, Hamster, Human, Chimpanzee, Rhesus monkey

    • 免疫原

      Synthetic peptide corresponding to Rat AANAT aa 195-205 (C terminal).
      Sequence:

      CHTFLRRNSGC


      (Peptide available as ab5004)
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • 常规说明

       This product was previously labelled as Serotonin N-acetyltransferase

       

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    性能

    • 形式

      Liquid

    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • 存储溶液

      Preservative: 0.05% Sodium azide
      Constituents: 0.1% BSA, 99% PBS
    • Concentration information loading...

    • 纯度

      Immunogen affinity purified

    • 纯化说明

      Total anti-C-terminal AA-NAT-specific IgG was affinity purified on resin-coupled non-phosphopeptide. Phospho-specific carboxy-terminal IgG was subsequently isolated on resin-coupled phosphopeptide.

    • Primary antibody说明

      Pineal gland serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase, AA-NAT) is an ~23 kDa protein which is the rate-limiting enzyme in melatonin synthesis. Melatonin serves as the hormonal signal of the daily light/dark cycle, or body clock. The nocturnal oscillation in enzyme activity ranges by 10- to 100-fold depending on species among vertebrates. Studies have shown that AA-NAT is regulated by cAMP at both the mRNA and protein levels. AA-NAT has putative protein kinase A (PKA) sites at Thr29 and Ser206. Phosphorylation of AA-NAT at these residues might play a role in protein inactivation via proteasome-dependent degradation.

    • 克隆

      多克隆

    • 同种型

      IgG

    • 研究领域

    应用

    Our Abpromise guarantee covers the use of ab3506 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 23 kDa.Can be blocked with AANAT peptide (ab5004).

    靶标

    • 功能

      Controls the night/day rhythm of melatonin production in the pineal gland. Catalyzes the N-acetylation of serotonin into N-acetylserotonin, the penultimate step in the synthesis of melatonin.

    • 组织特异性

      Highly expressed in pineal gland and at lower levels in the retina. Weak expression in several brain regions and in the pituitary gland.

    • 通路

      Aromatic compound metabolism; melatonin biosynthesis; melatonin from serotonin: step 1/2.

    • 疾病相关

      Delayed sleep phase syndrome

    • 序列相似性

      Belongs to the acetyltransferase family. AANAT subfamily.
      Contains 1 N-acetyltransferase domain.

    • 翻译后修饰

      cAMP-dependent phosphorylation on both N-terminal Thr-31 and C-terminal Ser-205 regulates AANAT activity by promoting interaction with 14-3-3 proteins.

    • 细胞定位

      Cytoplasm.
    • Information by UniProt

    • 数据库链接

      see all

    • 别名

      • AA-NAT antibody
      • Aanat antibody
      • Aralkylamine N-acetyltransferase antibody
      • Arylalkylamine N acetyltransferase antibody
      • Serotonin acetylase antibody
      • Serotonin N-acetyltransferase antibody
      • SNAT antibody
      • SNAT_HUMAN antibody
      see all

    图片

    • Western blot - Anti-AANAT antibody (ab3506)
      Western blot - Anti-AANAT antibody (ab3506)
      Western blot of Serotonin N-acetyltransferase in transfected 293T cell lysate using ab3506.

    文献 (1)

    发表研究结果有使用 ab3506？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab3506 被引用在 1 文献中.

    • Seo SH  et al. MicroRNA-150 controls differentiation of intraepithelial lymphocytes through TGF-ß receptor II regulation. J Allergy Clin Immunol 141:1382-1394.e14 (2018). PubMed: 28797734

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