A-431核extract裂解物(EGF treated) (ab14635)
概述
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产品名称
A-431核extract裂解物(EGF treated)
参阅全部 A431 细胞裂解液 -
常规说明
Cell line: A431 (Human epithelial carcinoma).
Cells were cultured in medium supplemented with 10 ng/mL EGF (epidermal growth factor) for 10 minutes at 37°C immediately prior to harvesting.
We recommend aliquoting the extracts into single use fractions and then storing.
性能
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Mycoplasma free
Yes -
形式
Liquid -
存放说明
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.90
Constituents: 0.75% Potassium chloride, 0.009% PMSF, 0.01% Magnesium chloride, 0.008% DTT, 0.48% HEPES, 20% Glycerol -
Concentration information loading...
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研究领域
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背景
A431 cells are a positive control for EGFR as they express high levels of EGFR. They also used for cell cycle studies and can be stimulated with EGF to stimulate EGFR and are therefore a very useful model to study EGFR downstream signalling cascades eg shc, src, MAPK.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab14635 尚未被引用在任何文献中。