概述

  • 产品名称
    Anti-A2B5抗体[105]
    参阅全部 A2B5 一抗
  • 描述
    小鼠单克隆抗体[105] to A2B5
  • 宿主
    Mouse
  • 经测试应用
    适用于: Flow Cyt, IHC-Fr, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Chicken, Human
  • 免疫原

    Embryonic chicken retinal cells.

  • 阳性对照
    • Type II astrocytes, human neural progenitors.
  • 常规说明

    This antibody clone is manufactured by Abcam.

     

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

性能

应用

Our Abpromise guarantee covers the use of ab53521 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µg for 106 cells.

ab18400 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.

 

IHC-Fr Use at an assay dependent concentration.
ICC/IF Use a concentration of 0.5 - 1 µg/ml.

靶标

  • 相关性
    A2B5 is a cell surface ganglioside epitope expressed in developing thymic epithelial cells, oligodendrocyte progenitors and neuroendocrine cells.
  • 细胞定位
    Cell surface

图片

  • ICC/IF image of ab53521 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53521, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgM (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).
  • A2B5 antibody [105] - Stem Cell Marker (ab53521) used in immunocytochemical detection on Cor1 neuronal stem cells. Cor1 neuronal stem cells were fixed in formaldehyde, permeabilized, blocked in 1% BSA for 30 mins at RT. ab53521 was incubated at 1/500 for 16 hours in TBS/BSA/azide/0.5% Triton. Secondary Antibody:anti mouse IgM conjugated: to Alexa Fluor® 488 (1/1000).

    See Abreview

  • Overlay histogram showing SH-SY5Y cells stained with ab53521 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53521, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgM (mu chain) (ab97007) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

文献

This product has been referenced in:
  • Naik AA  et al. Intra-generational protein malnutrition impairs temporal astrogenesis in rat brain. Biol Open 6:931-942 (2017). Read more (PubMed: 28546341) »
  • Tan B  et al. Effects of FTY720 (Fingolimod) on Proliferation, Differentiation, and Migration of Brain-Derived Neural Stem Cells. Stem Cells Int 2016:9671732 (2016). ICC/IF ; Rat . Read more (PubMed: 27829841) »
See all 29 Publications for this product

客户评价及客户问答

1-10 of 11 Abreviews or Q&A

Question
Answer

I have review the availible literature regarding the A2B5 epitope and have asked the lab to search as well. Unfortunately, we have found no instances recording the mRNA sequence for this target. A2B5 seems to have first been recognized in 1979 in by Eisenbarth et al, Proc. Nat. Acad. Sci. (1979, 76:4913–4917) and has been in use for some time as a marker for gangliosides in developing thymic epithelial cells, oligodendrocyte progenitors and neuroendocrine cells.

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Answer

Thank you for your inquiry.

Yes, it does appear these are the same product as they have the same clone number.

I hope this information helps. Please contact us with any other questions.

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Question
Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement of ab53521. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.  

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Answer

I am sorry this antibody is still not working for you. I will need the Abcam reference number for the order (six digits) or a PO number, or the date of the order and your institution. Did you have an antibody in mind that you would like to replace it with?

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Question
Answer

Thank you for bringing this issue to our attention. As I mentioned, if the PC12 stain does not work and you have confirmed that the secondary is appropriate (and that it is still fluorescent), I will replace the antibody. There is a chance that a single vial has gone bad. If you also consult the Bartholomeusz 1989 paper I mentioned and which you are already aware of, and any others you think may have an ICC protocol, you may find advice which we do not have on the datasheet.

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Question
Answer

I am very pleased to hear you would like to accept our offer and test ab66253 and ab53521 in IHC-P. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for IHC-P and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Brain)
Specification
Brain
Fixative
formalin
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH6
Permeabilization
No
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C

Abcam user community

Verified customer

提交于 Nov 22 2010

Answer

Thank you for your patience. The lab has finally isotyped the product and confirmed that it is a kappa isotype. Therefore, the suitable isotype control for this product is: ab18401 Mouse IgM, kappa monoclonal [MM-30] - isotype control Please let me know if there is anything else that I can help you with. Have a nice day!

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Answer

Thank you for your enquiry. Since the primary antibody is mouse IgM, you would need to use a secondary antibody that reacts with mouse IgM (anti-Mouse IgM or anti-Mouse IgG (Fab)). I am afraid that a mouse IgG might not suitable. I hope this information helps. If there is anything else that I can help you with, please do not hesitate to contact me.

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1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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