小鼠单克隆抗体[3424C1a] to ZNF75
Recombinant fragment comprising of 50-200 amino acids from the internal region of Human ZNF75 (NM_007131).
Recombinant fragment of human ZNF75.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, PBS, (8.0mM Sodium phosphate, 3.0mM Potassium chloride, 140mM Sodium chloride, 1.5mM Potassium phosphate), pH 7.4
Concentration information loading...
Protein G purified
ab76563 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 59 kDa. A band of 32 kDa was detected when the immunizing recombinant protein fragment was used.
Use at an assay dependent dilution.
May be involved in transcriptional regulation.
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 5 C2H2-type zinc fingers. Contains 1 KRAB domain. Contains 1 SCAN box domain.
Information by UniProt
Western blot - Anti-ZNF75 antibody [3424C1a] (ab76563)
Anti-ZNF75 antibody [3424C1a] (ab76563) at 2 µg/ml + immunizing recombinant protein
Predicted band size:
Observed band size:
32 kDa (
why is the actual band size different from the predicted?
The molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the protein was used.
has not yet been referenced specifically in any publications.
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