概述

  • 产品名称
  • 描述
    小鼠多克隆抗体to ZCCHC6
  • 宿主
    Mouse
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length human ZCCHC6 protein (aa 1-412 of AAH32456 or Swissprot id Q5VYS8-5).

  • 阳性对照
    • Human liver tissue lysate; ZCCHC6 transfected 293T cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab76901 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IHC-P
  • 应用说明
    IHC-P: 1/50.
    WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight, isoform 1: 171 kDa, isoform 5: 48 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性
      ZCCHC6 contains 3 CCHC-type zinc fingers.
    • 数据库链接
    • 别名
      • KIAA1711 antibody
      • MGC38585 antibody
      • PAP associated domain containing 6 antibody
      • PAPD6 antibody
      • Zinc finger CCHC domain containing 6 antibody
      see all

    图片

    • Anti-ZCCHC6 antibody (ab76901) at 5 µg/ml + human liver tissue lysate at 25 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

      Predicted band size: 171 kDa
      Observed band size: 150 kDa
      why is the actual band size different from the predicted?

    • All lanes : Anti-ZCCHC6 antibody (ab76901) at 5 µg/ml

      Lane 1 : ZCCHC6 transfected 293T cell lysate
      Lane 2 : Non transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      All lanes : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

      Predicted band size: 171 kDa
      Observed band size: 53 kDa why is the actual band size different from the predicted?



      Note: predicted molecular weight for isoform 5 is 48 kDa.
    • ab76901 staining ZCCHC6 in human breast cancer tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections). Tissue underwent antigen retrieval in 0.1 sodium citrate buffer. The primary antibody was used at 1/50 dilution and incubated with sample for 2 hours. The DAB staining method was used for detection. 

    文献

    ab76901 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    1-2 of 2 Abreviews or Q&A

    Answer

    Thank you for your message and for providing this further information. I am sorry to hear the suggestions made have not improved the results on this occasion. I appreciate the time you have spent on these experiments and would be pleased to arrange a replacement or a credit note in compensation. Please note that, unfortunately, we cannot offer an alternative catalog reference or another lot of ab76901. If your customer wants a replacement it will be from the same lot. I look forward to hearing from you with details of how you would like to proceed.

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    Question

    LOT NUMBER GR44511-2 ORDER NUMBER ********** DESCRIPTION OF THE PROBLEM Non-specific band SAMPLE Hela cell, whole cell lysate PRIMARY ANTIBODY Concentration or dilution : 1:100, 1:500 Diluent buffer : 1% Milk in PBS-T Incubation time : 2hr & Overnight Incubation temperature: 2hr at RT, Overnight at 4℃ Wash Buffer : PBST (0.1% Tween20) Number of washes 3 times DETECTION METHOD Chemidoc(Biorad) POSITIVE AND NEGATIVE CONTROLS USED - ANTIBODY STORAGE CONDITIONS -20C SAMPLE PREPARATION Lysis buffer : 200mM KCl, 20mM Tris, 0.2mM EDTA, 0.1% Triton-X100 Protease inhibitors: : Yes(Sigma PI cocktail) Phosphatase inhibitors : No Reducing agent : No Boiling for ≥5 min? yes/no : Yes AMOUNT OF PROTEIN LOADED 50ug/lane ELECTROPHORESIS/GEL CONDITIONS Fig1. 10% Fig2. 8% TRANSFER AND BLOCKING CONDITIONS Type of membrane : PVDF Protein transfer verified : WB same blot with another antibody  Yes Blocking agent and concentration : 5% Milk in PBS-T Blocking time : 1hr, Blocking temperature : RT(20℃) SECONDARY ANTIBODY Pierce 31430 Goat anti-mouse IgG(H+L) Concentration or dilution : 1:5000 Diluent buffer : PBST Incubation time : 40min, 1hr Incubation temperature: RT Fluorochrome or enzyme conjugate: HRP Wash Buffer : PBST (0.1% Tween20) Number of washes 3 times HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 3 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? Primary concentration, incubation time, incubation temperature ADDITIONAL NOTES -

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    Answer

    Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results. The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. Having reviewed this case, I would like to offer some suggestions to help optimize the results: When testing our antibodies, our lab uses 5% BSA as a blocking reagent, so I recommend switching to this instead of milk. Some antibodies give stronger, more specific signals on blots blocked with BSA instead of milk, so doing this may improve the results you are seeing, and reduce the non-specific bands. An example of the above is the western blot obtained with the Abcam GAPDH antibody ab9385 : Click here for the western blot image using ab9385 (or use the following: www.abcam.com/ab9385). Should the suggestions not improve the results, please do let me know. In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement or a credit note. I hope this information is helpful, and I thank you for your cooperation.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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