重组Anti-YY1抗体[EPR4652] - BSA and Azide free (ab232573)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4652] to YY1 - BSA and Azide free
- Suitable for: IHC-P, WB, ICC/IF, ChIC/CUT&RUN-seq, Flow Cyt (Intra)
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-YY1抗体[EPR4652] - BSA and Azide free
参阅全部 YY1 一抗 -
描述
兔单克隆抗体[EPR4652] to YY1 - BSA and Azide free -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, ICC/IF, ChIC/CUT&RUN-seq, Flow Cyt (Intra)more details
不适用于: ChIP or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- IHC-P: Human cervix carcinoma tissue.
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常规说明
ab232573 is the carrier-free version of ab109237.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.2
Constituent: PBS -
无载体
是 -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4652 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Anti-YY1 antibody [EPR4652] - Nuclear Loading Control (ab109237)
- Alexa Fluor® 488 Anti-YY1 antibody [EPR4652] - Nuclear Marker (ab199814)
- PE Anti-YY1 antibody [EPR4652] (Nuclear Loading Control) (ab305761)
- APC Anti-YY1 antibody [EPR4652] (Nuclear Loading Control) (ab305762)
- HRP Anti-YY1 antibody [EPR4652] (Nuclear Loading Control) (ab305763)
- Alexa Fluor® 647 Anti-YY1 antibody [EPR4652] - Nuclear Loading Control (ab310147)
- Alexa Fluor® 594 Anti-YY1 - Nuclear Loading Control antibody [EPR4652] (ab310573)
- Alexa Fluor® 555 Anti-YY1 antibody [EPR4652] - Nuclear Loading Control (ab312103)
- Alexa Fluor® 568 Anti-YY1 antibody [EPR4652] - Nuclear Loading Control (ab312584)
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab232573于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 45 kDa.
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ICC/IF |
Use at an assay dependent concentration.
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ChIC/CUT&RUN-seq |
Use at an assay dependent concentration.
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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说明 |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 45 kDa. |
ICC/IF
Use at an assay dependent concentration. |
ChIC/CUT&RUN-seq
Use at an assay dependent concentration. |
Flow Cyt (Intra)
Use at an assay dependent concentration. |
靶标
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功能
Multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. May play an important role in development and differentiation. The function of YY1 as an activator or a repressor is specified by the presence of other proteins. For example it acts as a repressor in absence of adenovirus E1A protein but as an activator in its presence. -
序列相似性
Belongs to the YY transcription factor family.
Contains 4 C2H2-type zinc fingers. -
细胞定位
Nucleus matrix. Associated with the nuclear matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 7528 Human
- Entrez Gene: 22632 Mouse
- Entrez Gene: 24919 Rat
- Omim: 600013 Human
- SwissProt: P25490 Human
- SwissProt: Q00899 Mouse
- Unigene: 388927 Human
- Unigene: 3868 Mouse
see all -
别名
- CF1 antibody
- Delta antibody
- Delta transcription factor antibody
see all
图片
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This data was developed using the same antibody clone in a different buffer formulation (ab109237).
ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/µL, 2.5 x 10^5 K-562 (Human chronic myelogenous leukemia lymphoblast) cells and 5 µg of ab109237 [EPR4652]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods. -
This data was developed using the same antibody clone in a different buffer formulation (ab109237).
ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/µL, 2.5 x 10^5 K-562 (Human chronic myelogenous leukemia lymphoblast) cells and 5 µg of ab109237 [EPR4652]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods. -
This data was developed using the same antibody clone in a different buffer formulation (ab109237).
ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/µL, 2.5 x 10^5 K-562 (Human chronic myelogenous leukemia lymphoblast) cells and 5 µg of ab109237 [EPR4652]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods. -
ab109237 staining YY1 in the human cell line HeLa (human cervix adenocarcinoma) by intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde, permiabilised with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/30. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.
Isoytype control: Rabbit monoclonal IgG (Black)
Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109237).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervix carcinoma tissue labelling YY1 with purified ab109237 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109237).
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Immunocytochemistry/Immunofluorescence analysis of HUT-78 cells labelling YY1 with purified ab109237 at 1/50. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.
Control: primary antibody (1/50) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109237).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis human kidney tissue labelling YY1 with unpurified ab109237 at 1/250.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109237).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis human tonsil tissue labelling YY1 with unpurified ab109237 at 1/250.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109237).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (1)
ab232573 被引用在 1 文献中.
- Cheng Q et al. Overexpression of CD36 in mammary fibroblasts suppresses colony growth in breast cancer cell lines. Biochem Biophys Res Commun 526:41-47 (2020). PubMed: 32192771