Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Schlemms Canal Endothelium)
Specification
Schlemms Canal Endothelium
Fixative
Formaldehyde
Permeabilization
Yes - 0.2% Triton X-100
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Other product details
Dilution
1/200
Incubation time
3 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: DPBS
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-rabbit IgG (H+L) 2 mg/ml
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Thomas Read
Verified customer
提交于 Oct 31 2011