Application
Western blot
Sample
Mouse Cell lysate - other (COS cells)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
20 µg
Specification
COS cells
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: milk
Dilution
1/2000
Secondary antibody
Dilution
1/20000
Name
Non-Abcam antibody was used: GARPO
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Negative control
empty vector
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 90 kDa
Positive control
overexpression
Additional data
Additional Notes
This antibody works very well and contributed greatly to our recent experiments.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Oct 27 2016