参阅全部 UCH37 一抗
描述兔单克隆抗体[EPR4896] to UCH37
经测试应用适用于: WB, IP, IHC-P, Flow Cytmore details
种属反应性与反应: Rat, Human
Synthetic peptide within Human UCH37 aa 1-100. The exact sequence is proprietary.
- Human fetal kidney, 293T, NCCIT and SH-SY5Y cell lysates; Human kidney tissue.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
纯度Tissue culture supernatant
Our Abpromise guarantee covers the use of ab124931 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 38 kDa).|
|IP||1/10 - 1/100.|
|IHC-P||1/500 - 1/1000. Antigen retrieval is recommended. Heat up to 98 °C, below boiling, and then let cool for 10-20 min.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
功能Protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains. Deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome.
序列相似性Belongs to the peptidase C12 family.
细胞定位Cytoplasm. Nucleus. Associates with the proteasome 19S subunit in the cytoplasm. Associates with the INO80 complex in the nucleus.
- Information by UniProt
- UCH37 antibody
- AD-019 antibody
- CGI 70 antibody
All lanes : Anti-UCH37 antibody [EPR4896] (ab124931) at 1/1000 dilution
Lane 1 : Human fetal kidney cell lysates
Lane 2 : 293T cell lysates
Lane 3 : NCCIT cell lysates
Lane 4 : SH-SY5Y cell lysates
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 38 kDa
ab124931, at 1/500 dilution, staining UCH37 in paraffin embedded Human kidney tissue by Immunohistochemistry.
This product has been referenced in:
- Liao Y et al. Growth arrest and apoptosis induction in androgen receptor-positive human breast cancer cells by inhibition of USP14-mediated androgen receptor deubiquitination. Oncogene 37:1896-1910 (2018). Human . Read more (PubMed: 29353883) »
- Lee JH et al. Inactivation of USP14 Perturbs Ubiquitin Homeostasis and Delays the Cell Cycle in Mouse Embryonic Fibroblasts and in Fruit Fly Drosophila. Cell Physiol Biochem 47:67-82 (2018). Read more (PubMed: 29763934) »