Triglyceride Assay试剂盒- Quantification  (ab65336)

概述

  • 产品名称
    Triglyceride Assay试剂盒- Quantification 
    参阅全部 Triglyceride 试剂盒
  • 样品类型
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue, Adherent cells, Suspension cells
  • 检测类型
    Quantitative
  • 灵敏度
    > 2 µM
  • 范围
    2 µM - 10000 µM
  • 检测时间
    1h 00m
  • 产品概述

    Triglyceride Quantification Assay Kit (ab65336) provides a sensitive, easy assay to measure triglyceride (TG) concentration in mammalian samples. In the assay, TG are converted to free fatty acids and glycerol. The glycerol is then oxidized to generate a product which reacts with the probe to generate color (spectrophotometry at λ= 570 nm) and fluorescence (Ex/Em = 535/587 nm).


    If your sample contains reducing substances such as glucose, fructose or lactose, they are likely to interfere with the assay. In this case, we recommend using PicoProbe Triglyceride Quantification Assay Kit (Fluorometric) (ab178780).


    Visit our FAQs page for tips and troubleshooting.


    Review our Metabolism Assay Guide to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader.

  • 说明

    This kit also detects monoglycerides and diglycerides.

    Some components of this kit are available for purchasing as standalone reagents if you need more quantities (lipase, enzyme mix, TG standard or TG probe). Please contact our team for more details.

性能

图片

  • Hepatic triglyceride levels was measured using ab65336 in male and female wild-type (WT) or AT2KO (knockout) mice with either normal diet (ND) or high fat diet (HFD). 

  • Fluorometric triglyceride standard curve: mean of duplicates (+/- SD) with background reads subtracted

  • Triglyceride measured in cell culture lysates showing quantity (nmol) per 1 mln cells.

    Samples with the concentration of 1e7 cells/mL were used. Samples were diluted 40-80 fold and measured fluorometrically.

    HepG2 cells were treated with 25 uM Chloroquine for 72h.

  • Colorimetric triglyceride standard curve using ab65336.

实验方案

文献

This product has been referenced in:
  • García-Ruiz I  et al. Omentectomy Prevents Metabolic Syndrome By Reducing Appetite and Body Weight In A Diet-Induced Obesity Rat Model. Sci Rep 8:1540 (2018). WB . Read more (PubMed: 29367725) »
  • Feng B  et al. Hypothalamic POMC expression is required for peripheral insulin action on hepatic gluconeogenesis through regulating STAT3 in sepsis rats. J Cell Mol Med 22:1696-1707 (2018). Read more (PubMed: 29285858) »

See all 67 Publications for this product

客户评价及客户问答

We prefer NP40 to Triton or tween in this assay. NP-40 works better to keep lipids in solution and does not create background in the assay.

The picoprobe allows ab178780 to be more sensitive than the ab65336. ab178780 detects triglycerides <0.4uM whereas ab65336 can detect 2uM-10mM triglycerides.

I hope this helps!

The lipase is from a bacterial source and the standards are from plant oils.

Yes, I can confirm that there should be no problem to treat the plasma samples in the same way as the serum, directly applying it.

1) The insoluble top layer may contain the triglyceride you wish to measure so please make sure to solubilize as much as possible (without losing any material) and to follow the recommendations (ie. repeated heating steps).
2) SV is indeed the abb...

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The 96 well plate is not provided with the kit. Please use a black plate (clear bottom) for fluorescence and clear plate for colorimetric analysis.

The standard provided with ab65336 has a molecular weight if 885.43 g/mol.

1 mol/L is represented as 1mM, E.g. 50 mM is 50 mmol/L.

Divide by the molecular weight of the substance (average triglyceride molecular weight in your sample) to convert from moles to grams. Then mM/mol weight can be converted to get mg/L. Read More

Comparing a serial dilution of yellow-bellied marmot (Marmota flaviventris) to a standard serial dilution, we determined the quantity of plasma to use in subsequent assays (shown only the greatest dilution fell on the curve).
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Verified customer

提交于 Jul 22 2016

I would recommend to use more than 1 ml NP-40/water solution to process the fattier tissue. You can also heat for >5 mins to make sure that all the TG has been extracted. For the colorimetric assay, having fat globules in the sample solution in the ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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