Application
Western blot
Sample
Mouse Cell lysate - whole cell (Testes)
Gel Running Conditions
Reduced Non-Denaturing (Native) (NuPage 4-12% Bis-Tris)
Loading amount
1 µg
Specification
Testes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk
Dilution
1/2500
Secondary antibody
Dilution
1/4000
Name
Non-Abcam antibody was used: Jackson ImmunoResearch Peroxidase-conjugated Affin
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
Amersham ECL
Exposure
5 minute(s) and 0 second(s)
Positive control
Three whole cell lysates from testes.
Additional data
Additional Notes
Antibody showed heavy background (not specific to secondary) and white regions indicating over exposure at 5 minutes. Background is heavy and black in between protein lanes and at less than 20kDa, and white within lanes.
1:2500 exposure was tried after 1:750 showed even heavier background.
Abcam response
Thank you so much for testing this antibody in mouse and WB.
We appreciate very much your efforts, but are sorry to hear that the antibody did not work in this species and application. The customer received a discount on a future antibody purchase for testing this product in mouse and WB. We would welcome additional feedback from other customers as well.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Feb 08 2013