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The transmembrane protein Tissue factor (TF) is the physiologic trigger of coagulation in normal hemostasis. Tissue factor binds and allosterically activates factor VII (FVII). The TF-FVIIa complex cleaves factor IX and X, leading to thrombin generation. Tissue factor markedly enhances the ability of FVIIa to cleave both macromolecule and small peptidyl substrates. Inducible expression of Tissue factor in a variety of pathological conditions, including gram-negative sepsis and acute coronary syndromes, is associated with life-threatening thrombosis. In sepsis, Tissue factor expression within the vasculature leads to disseminated intravascular coagulation. Tissue factor also plays important roles in vasculogenesis, metastasis, and tumor-associated angiogenesis.
ab108906 Tissue Factor Human Chromogenic Activity Assay kit is developed to determine Human Tissue Factor activity in plasma, tissue and cell culture supernatants. The assay measures the ability of lipoprotein TF/FVIIa to activate factor X (FX) to factor Xa. The amidolytic activity of the TF/FVIIa complex is quantitated by the amount of FXa produced using a highly specific FXa substrate releasing a yellow para-nitroaniline (pNA) chromophore. The change in absorbance of the pNA at 405 nm is directly proportional to the TF enzymatic activity.
|Assay Diluent||1 x 20ml|
|FXa Substrate||2 units|
|Human FVII||1 unit|
|Human FX||1 unit|
|rh Tissue Factor Standard (lipoprotein)||1 unit|
|Sample Diluent||1 x 11ml|
|Sealing Tapes||3 units|
|Tissue Factor Microplate (12 strips of 8 wells)||1 unit|
Colorimetric standard curve: mean of duplicates (+/- SD)
Standard curve showing concentration (pM) on X-axis and increase of O.D. per min on Y-axis (time frame: 20 min.)
Time course showing O.D.'s of one standard point and one sample (undiluted vs.diluted)
Tissue Factor measured in various biologicals showing average concentrations
ab108906 has not yet been referenced specifically in any publications.
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