概述

  • 产品名称
    Anti-Thymidine Kinase 1抗体[EPR3194]
    参阅全部 Thymidine Kinase 1 一抗
  • 描述
    兔单克隆抗体[EPR3194] to Thymidine Kinase 1
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, Flow Cyt, ICC/IFmore details
    不适用于: IHC-P or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (Human)

  • 阳性对照
    • Hela cells and Molt-4 cell lysate
  • 常规说明

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • 纯度
    Tissue culture supernatant
  • 克隆
    单克隆
  • 克隆编号
    EPR3194
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab91651 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 25 kDa.
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100 - 1/250.
  • 应用说明
    Is unsuitable for IHC-P or IP.
  • 靶标

    • 序列相似性
      Belongs to the thymidine kinase family.
    • 翻译后修饰
      Phosphorylated on Ser-13 in mitosis.
    • 细胞定位
      Cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
      • cytosolic antibody
      • KITH_HUMAN antibody
      • Thymidine kinase 1 antibody
      • Thymidine kinase 1 soluble antibody
      • Thymidine kinase 1 soluble isoform antibody
      • Thymidine kinase antibody
      • Thymidine kinase cytosolic antibody
      • TK 1 antibody
      • TK 2 antibody
      • TK1 antibody
      • Tk1a antibody
      • Tk1b antibody
      • TK2 antibody
      see all

    图片

    • Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
      Lane 2: TK1 knockout HAP1 whole cell lysate (20 µg)
      Lane 3: Jurkat whole cell lysate (20 µg)
      Lane 4: MOLT4 whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab91651 observed at 25 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab91651 was shown to specifically recognize TK1 in wild-type HAP1 cells along with additional cross-reactive bands. No bands were observed when TK1 knockout samples were examined. Wild-type and TK1 knockout samples were subjected to SDS-PAGE.  Ab91651 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/500 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.

    • ab91651 at 1/100 dilution staining Thymidine kinase 1 in Hela cells
    • Anti-Thymidine Kinase 1 antibody [EPR3194] (ab91651) at 1/500 dilution + Molt-4 cell lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 25 kDa

    文献

    This product has been referenced in:
    • Weagel EG  et al. Biomarker analysis and clinical relevance of TK1 on the cell membrane of Burkitt's lymphoma and acute lymphoblastic leukemia. Onco Targets Ther 10:4355-4367 (2017). Read more (PubMed: 28919785) »
    See 1 Publication for this product

    客户评价及客户问答

    1-3 of 3 Abreviews or Q&A

    Answer

    Thank you for your enquiry.

    I'm sorry to confirm this antibody is not tested in IHC on paraffin sections (IHC-P). All tested species and applications covered by the 6 month guarantee are listed on the individual datasheets. These are updated as soon as we have any new information.

    The antibody has also been tested unsuccessfully in mouse, as stated on the datasheet. Therefore, regrettably this is not the most suitable antibody for your experiments.

    I can suggest one of the following two, tested in IHC-P and mouse, would be more suitable and will be covered by our 6 month guarantee in this application and species. I can recommend to review the online datasheets for further details:

    ab59271 Anti-Thymidine Kinase 1 antibody
    Tested in: IHC-P, WB, ELISA
    Rabbit polyclonal
    Reacts with:Human, Mouse
    https://www.abcam.com/index.html?datasheet=59271 (or use the following: https://www.abcam.com/index.html?datasheet=59271).

    ab76495 2563-1 Anti-Thymidine Kinase 1 antibody [EPR3193]
    Tested in: Flow Cyt, IP, WB, ICC/IF, IHC-P
    Rabbit monoclonal
    Reacts with: Human, Mouse
    https://www.abcam.com/index.html?datasheet=76495 (or use the following: https://www.abcam.com/index.html?datasheet=76495).

    According to the EST expression profile on the Unigen database link on the datasheet, in mouse the Tk1 protein is expressed quite highly in lymph node and several other tissues.
    http://www.ncbi.nlm.nih.gov/UniGene/ESTProfileViewer.cgi?uglist=Mm.2661

    I can therefore recommend to consider lymph node as a positive control tissue. You may wish to do some further literature searching for more information.

    Read More

    Answer

    Thank you for contacting Abcam.

    The main difference between ab76495 and ab91651 is that they will have different epitope recognition sites.

    Also for ab76495, we guarantee it to react with mouse and human tissue in the following applications, ICC/IF, WB, IP, IHC-P, Flow Cyt. Whereas ab91651 will react with human tissue but not mouse and rat and will work in the following applications WB, Flow Cyt, ICC/IF. Therefore ab91651 would not work for you since you wish to use the antibody on mouse sections.

    If there is anything else I can help you with, or if you need further selecting the most appropriate antibody for your experiments, please let me know.

    Read More

    Answer

    Thank you very much for your enquiry. Based on the immunogen sequences both Thymidine Kinase 1 (TK1) antibodies ab76495 and ab91651 are specific for TK1 and will not recognise TK2: The immunogen sequence for ab76495 is a peptide near the N-terminus (amino acids a-b) of human TK1 (SwissProt P04183), whereas ab91651 has its immunogen located at the C-terminus (amino acids y-z). These sequences do not share any homology with TK2. The TK1 antibody ab57757 has not been tested for a potential cross-reactivity with TK2. However, the immunogen is the full-length human TK1 which reveals no significant sequence similarity with human TK2 (SwissProt O00142). We therefore would not expect this antibody to cross-react with TK2. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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