Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Sample
Pig Tissue sections (liver)
Specification
liver
Permeabilization
No
Fixative
Paraformaldehyde
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: antibody reagens Fa.Zytomed
Dilution
1/200
Secondary antibody
Name
Non-Abcam antibody was used: ABC Fa.Zytomed
Host species: Pig
Clonality: Polyclonal
Conjugation: Biotin
Host species: Pig
Clonality: Polyclonal
Conjugation: Biotin
Additional data
Additional Notes
Broad spectrum streptavidin-biotin system used for detection. Able to detect primary antibodies raised in mouse, rabbit, rat and guinea pig.
Abcam response
Thank you for sharing your results with us. This antibody should recognise porcine Thrombospondin. We would suggest running a good positive control (sections of human tonsils or glioblastoma) alongside with the samples and checking the detection system.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Nov 29 2013