Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Human Tissue sections (Tau/APP transgenic mouse brain expressing human Ta)
Specification
Tau/APP transgenic mouse brain expressing human Ta
Permeabilization
No
Fixative
Formaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide
Dilution
1/2000
Secondary antibody
Name
Non-Abcam antibody was used: anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/250
Additional data
Additional Notes
Submitted composite image of Frontal cortex shows no obvious neurones with NFTs (upper image) whereas they are clearly seen using another antibody ( lower image). Both images are near serial sections from a Tau/APP transgenic mouse.
Abcam response
Thank you for you valuable feedback. It appears that under these experimental conditions ab109390 does not specifically detect human Tau phosphorylated at Serine 396 in transgenic mice. We welcome further customer comments. For more information, please contact scientific support.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Carl Hobbs
Verified customer
提交于 Dec 12 2013