Application
Western blot
Sample
Human Cell lysate - whole cell (MDA-MB-231)
Gel Running Conditions
Reduced Denaturing (7.5%)
Loading amount
50 µg
Specification
MDA-MB-231
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000
Secondary antibody
Dilution
1/15000
Name
Non-Abcam antibody was used: anti-Rabbit IR Dye 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Detection
Detection method
LiCor
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 55 kDa Non-specific: 300 kDa
Positive control
cell lysate
Additional data
Additional Notes
NC membrane
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Aug 26 2015