Application
Western blot
Sample
Rat Tissue lysate - other (brain, HEK cell lysate)
Loading amount
50 µg
Specification
brain, HEK cell lysate
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Other product details
Dilution
1/1000
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST milk
Secondary antibody
Name
Non-Abcam antibody was used: rabbit anti-mouse hrp antibody
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
femto, pierce
Exposure
30 second(s)
Bands
Specific: 47 kDa Non-specific: 70 kDa
Additional data
Additional Notes
1 = Post-nuclear P21 rat brain extract
2 = Hek cells transfected with control siRNA
3 = Hek cells transfected with TSG101 siRNA
2 = Hek cells transfected with control siRNA
3 = Hek cells transfected with TSG101 siRNA
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Sriharsha Kantamneni
Verified customer
提交于 Sep 26 2007