Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (embryonic stem cell)
Permeabilization
No
Specification
embryonic stem cell
Blocking step
BSA as blocking agent for 40 minute(s) · Concentration: 2% · Temperature: 22°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: block
Dilution
1/100
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: Alexa fluor 488 goat anti-mouse IgG
Host species: Mouse
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Mouse
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Abcam response
Thank you for submitting your Abreview. We are very sorry to hear that the antibody did not perform as expected in this tested species and application. The absence of signal may be due to the lot used having been purchased 6-7 years ago.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Kate Hawkins
Verified customer
提交于 May 17 2016