参阅全部 TPA Tissue Plasminogen Activator 一抗
特异性There were no cross reactivities obtained with human uPA and PAI2.
经测试应用适用于: ELISA, IHC-Fr, IHC-Pmore details
种属反应性与反应: Rat, Human
Full length native protein (purified) (Human).
存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
存储溶液Constituent: Whole serum
Our Abpromise guarantee covers the use of ab14198 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent dilution.|
|IHC-Fr||1/50 - 1/100.|
|IHC-P||1/50. Pretreatment with 0.05% Pronase E for 20 minutes is recommended.|
功能Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. Play a direct role in facilitating neuronal migration.
组织特异性Synthesized in numerous tissues (including tumors) and secreted into most extracellular body fluids, such as plasma, uterine fluid, saliva, gingival crevicular fluid, tears, seminal fluid, and milk.
疾病相关Note=Increased activity of TPA results in increased fibrinolysis of fibrin blood clots that is associated with excessive bleeding. Defective release of TPA results in hypofibrinolysis that can lead to thrombosis or embolism.
序列相似性Belongs to the peptidase S1 family.
Contains 1 EGF-like domain.
Contains 1 fibronectin type-I domain.
Contains 2 kringle domains.
Contains 1 peptidase S1 domain.
结构域Both FN1 and one of the kringle domains are required for binding to fibrin.
Both FN1 and EGF-like domains are important for binding to LRP1.
The FN1 domain mediates binding to annexin A2.
The second kringle domain is implicated in binding to cytokeratin-8 and to the endothelial cell surface binding site.
翻译后修饰The single chain, almost fully active enzyme, can be further processed into a two-chain fully active form by a cleavage after Arg-310 catalyzed by plasmin, tissue kallikrein or factor Xa.
Differential cell-specific N-linked glycosylation gives rise to two glycoforms, type I (glycosylated at Asn-219) and type II (not glycosylated at Asn-219). The single chain type I glycoform is less readily converted into the two-chain form by plasmin, and the two-chain type I glycoform has a lower activity than the two-chain type II glycoform in the presence of fibrin.
N-glycosylation of Asn-152; the bound oligomannosidic glycan is involved in the interaction with the mannose receptor.
Characterization of O-linked glycan was studied in Bowes melanoma cell line.
细胞定位Secreted > extracellular space.
- Information by UniProt
- Alteplase antibody
- DKFZp686I03148 antibody
- Plasminogen activator tissue antibody
a14198 staining TPA Tissue Plasminogen Activator (A - blue) in Rat hippocampal/thalamic region tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde. Tissue was incubated in pre-block solution (10% normal hourse serum in antibody diluent (PBS + 0.1% Trition X-100)) for 1 hour. Samples were incubated with primary antibody (1/50 in PBS + pre-block solution) for 16 hours. B - trypsin (red), C - PAR2 (green), D - merge.
TPA Tissue Plasminogen Actvator and Trypsin co-localise.
ab14198 at 1/50 staining rat brain hippocampus pyramidal neuron tissue sections by IHC-Fr. The tissue was blocked with horse serum and incubated for 16 hours with the antibody. An AMCA conjugated donkey antibody was used as the secondary.
This product has been referenced in:
- Mauprivez C et al. Periosteum Metabolism and Nerve Fiber Positioning Depend on Interactions between Osteoblasts and Peripheral Innervation in Rat Mandible. PLoS One 10:e0140848 (2015). Read more (PubMed: 26509533) »
- Granieri L et al. A competition-based assay for the screening of species-specific antibiotics. J Antimicrob Chemother 64:62-8 (2009). IF ; Human . Read more (PubMed: 19401303) »