Application
Immunoprecipitation
Sample
Mouse Cell lysate - whole cell (NIH3T3 Mouse Fibroblasts)
Total protein in input
100 µg
Specification
NIH3T3 Mouse Fibroblasts
Immuno-precipitation step
Protein A/G
Other product details
Dilution
1/200
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Abcam 1x ChIP buffer (ab500)
Western Blot antibody
Name
Abcam antibody:Anti-TLR5 antibody [19D759.2]
Concentration
1 µg/mg lysate
Additional data
Additional Notes
TLR5 antibody (ab13876) was also used for Western blot to confirm successful IP
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提交于 Jan 11 2011