Application
Flow Cytometry
Sample
Mouse Cell (NIH 3T3 Mouse Fibroblasts)
Specification
NIH 3T3 Mouse Fibroblasts
Preparation
Cell harvesting/tissue preparation method: Trypsin/centrifugation
Sample buffer: PBS 5%FCS
Sample buffer: PBS 5%FCS
Fixation
Formaldehyde
Permeabilization
Yes - 0.025% Triton-X in PBS
Gating Strategy
Size gated NIH 3T3
Other product details
Concentration
2 µg/cells
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 23°C · Diluent: PBS 5%FCS
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-Mouse Alexa Fluor 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/1000
Additional data
Additional Notes
Used at cincentration of 2ug/10^6 cells
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Sep 23 2010