Application
Flow Cytometry
Sample
Human Cell (SW480, SW620 (colon cancer) and Fadu, Detroit 562)
Specification
SW480, SW620 (colon cancer) and Fadu, Detroit 562
Preparation
Cell harvesting/tissue preparation method: Cells were trypsinized and washed in PBS.
Sample buffer: 2% FBS/0.1%Na-azide/PBS
Sample buffer: 2% FBS/0.1%Na-azide/PBS
Fixation
Paraformaldehyde
Permeabilization
Yes - 0.2% tween
Gating Strategy
There was only one population visible after the staining. Gating and analysis was performed by WinMDI 2.9 software.
Other product details
Concentration
1 µg/cells
Incubation time
30 minute(s) · Temperature: 4°C · Diluent: PBS, 2% FBS and 0.1% Na-azide
Secondary antibody
Name
Non-Abcam antibody was used: Dako, Glostrup, Denmark
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Fluorescein
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Fluorescein
Dilution
1/40
Additional data
PubMed ID
19522763: View
Additional Notes
Figure 1. A representative fluorescence-activated cell sorter (FACS) analysis of TLR3 protein expression in SW480 (A), SW620 (B), FaDu (C) and Detroit 562 (D) cell lines. Cell lines were permeabilized and immunostained with TLR3-specific antibody (thick black line) or isotype-control antibody (filled grey area). Fluorescence tracings are representative of three independent experiments. SW620 and Detroit 562 cells were stained with 1 µg, SW480 with 1.5 µg and FaDu with 2 µg of TLR3 antibody and isotope control respectively.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mrs. Tanja Matijevic Glavan
Verified customer
提交于 Jul 14 2009