存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Constituents: PBS, pH 7.2
Concentration information loading...
纯度Protein A purified
Our Abpromise guarantee covers the use of ab72624 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 133 kDa).|
功能Tubulin-folding protein; involved in the first step of the tubulin folding pathway. Modulates microtubule dynamics by capturing GTP-bound beta-tubulin (TUBB). Acts as a GTPase-activating protein (GAP) for ARL2. Its ability to interact with beta tubulin is regulated via its interaction with ARL2. Induces microtubule disruption in absence of ARL2. Increases degradation of beta tubulin, when overexpressed in polarized cells. Promotes epithelial cell detachment, a process antagonized by ARL2. Induces tight adherens and tight junctions disassembly at the lateral cell membrane.
序列相似性Belongs to the TBCD family.
Contains 3 HEAT repeats.
细胞定位Cell junction > tight junction. Lateral cell membrane. Cytoplasm. Cell junction > adherens junction. Localized in cell-cell contacts.
- Information by UniProt
- Beta tubulin cofactor D antibody
- Beta-tubulin cofactor D antibody
- KIAA0988 antibody
All lanes : Anti-TBCD antibody (ab72624) at 1/500 dilution
Lane 1 : TBCD transfected 293T cell lystae
Lane 2 : Non transfected 293T cell lystae
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-Mouse IgG(H&L)-HRP Conjugate at 1/25000 dilution
Predicted band size: 133 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
ab72624 has not yet been referenced specifically in any publications.