Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Antigen retrieval step
None
Sample
Mouse Tissue sections (Brain sections)
Specification
Brain sections
Permeabilization
No
Fixative
Paraformaldehyde
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: PBS + 0.3 % Triton X100
Dilution
1/3000
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit Alexa Fluor 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/1000
Additional data
Additional Notes
The antibody produced a strong and diffuse staining in the whole mouse brain (diluted 1:3000). The picture submitted shows the staining obtained at the level of the cortex.
The tissues were fixed (animals perfused fixed) with 4% PFA and later postfixed overnight in the same fixative. They were cryoprotected in 30% sucrose and cut using a cryostat.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Sophie Pezet
Verified customer
提交于 Jun 17 2009