Anti-Strep-tag II抗体(ab76950)
Key features and details
- Rabbit polyclonal to Strep-tag II
- Suitable for: WB
- Reacts with: Species independent
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Strep-tag II抗体
参阅全部 Strep-tag II 一抗 -
描述
兔多克隆抗体to Strep-tag II -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Species independent -
免疫原
Amino acid sequence gggWSHPQFEK.
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阳性对照
- Streptavidin tagged recombinant protein
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Affinity chromatography purified via peptide column -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab76950于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 24 kDa.
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说明 |
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WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 24 kDa. |
靶标
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相关性
Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag (AWRHPQFGG) was a nine amino acid peptide with high specificity and affinity towards streptavidin which allows the simple purification of protein by use of affinity columns, but required addition to only the C-terminus of recombinant proteins. To also allow a Strep-tag to be placed at the N-terminus of recombinant proteins, it was re-engineered and re-named Strep-tag II. -
别名
- anti-Strep-Tag II antibody
- Strep-Tag II antibody
- Strep-Tag II antibody antibody
- Strep-Tag IIantiboy antibody
图片
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All lanes : Anti-Strep-tag II antibody (ab76950) at 1 µg/ml
Lane 1 : Streptavidin tagged recombinant protein with blocking peptide
Lane 2 : Streptavidin tagged recombinant protein
Lysates/proteins at 0.05 µg per lane.
Predicted band size: 24 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab76950 被引用在 4 文献中.
- Reichmann J et al. Tex19.1 inhibits the N-end rule pathway and maintains acetylated SMC3 cohesin and sister chromatid cohesion in oocytes. J Cell Biol 219:N/A (2020). PubMed: 32232464
- Long T et al. Structural basis for human sterol isomerase in cholesterol biosynthesis and multidrug recognition. Nat Commun 10:2452 (2019). PubMed: 31165728
- Baggett NE et al. Global analysis of translation termination in E. coli. PLoS Genet 13:e1006676 (2017). PubMed: 28301469
- Lin Y et al. Antiaging Gene Klotho Deficiency Promoted High-Fat Diet-Induced Arterial Stiffening via Inactivation of AMP-Activated Protein Kinase. Hypertension 67:564-73 (2016). PubMed: 26781278