Application
Western blot
Sample
Human Cell lysate - other (cell lysate containing expressed recombinant prote)
Loading amount
25 µg
Specification
cell lysate containing expressed recombinant prote
Gel Running Conditions
Reduced Denaturing (Bis-Tris 4-20%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Dilution
1/2000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS with 0.5% milk and 0.1% Tween-20
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-rabit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000
Detection
Detection method
ECL
Exposure
30 second(s)
Bands
Specific: 68 kDa
Negative control
cell lysate from cells that do not express the recombinant strep-tagged protein
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Dec 03 2012