Application
ChIP
Detection step
Real-time PCR
Sample
Human Cell lysate - nuclear (MCF7 breast cancer cell line)
Specification
MCF7 breast cancer cell line
Negative control
MCF7 IgG
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Positive control
MCF7 H3
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 25mM Tris, 150mMNaCl 3mM EDTA 1% Triton
Additional data
Additional Notes
I have not used the SceI primers for qPCR after ChIP
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Adina Brett-Morris
Verified customer
提交于 Nov 12 2013