Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa cells)
Specification
HeLa cells
Fixative
Formaldehyde
Permeabilization
No
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 27°C
Other product details
Dilution
1/100
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 27°C
Secondary antibody
Name
Non-Abcam antibody was used: Molecular Probe Donkey anti Rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Dilution
1/1000
Additional data
Additional Notes
We tried this antibody for immunofluorescence with three different fixations - 4% formadehyde at RT for 15 min, MeOH on ice for 3 min, and 10% TCA on ice for 10% (image not shown). All three fixations gave us similar results. Signal was recognize in cytoplasm but not in nucleus. Samples handled without Ab26975 as primary antibody were used as negative controls.
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提交于 Sep 09 2010