Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (embryo heart)
Permeabilization
Yes - 0.3% Triton
Specification
embryo heart
Blocking step
Normal Donkey Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Paraformaldehyde
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Normal Donkey serum in PBS-tween
Dilution
1/100
Secondary antibody
Dilution
1/400
Name
Non-Abcam antibody was used: anti rabbit alexa fluor 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Abcam response
Thank you so much for testing this antibody in IHC-Fr. We appreciate very much your efforts, but are sorry to hear that the antibody did not work well in this application.
We welcome additional feedback from other customers as well.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Mar 07 2018