Application
Western blot
Sample
Rat Tissue lysate - whole (Penile Tissue)
Loading amount
80 µg
Specification
Penile Tissue
Gel Running Conditions
Reduced Denaturing (4-20% gel)
Blocking step
Starting Block T20 (PBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
22 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA/TBST
Secondary antibody
Name
Non-Abcam antibody was used: ECL Anti-mouse IgG
Host species: Sheep
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Sheep
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/3000
Detection
Detection method
Chemiluminescence, ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 60 kDa
Positive control
i did not have a positive control
Negative control
i did not have a negative control
Additional data
Additional Notes
I used 3% BSA/TBST to dilute my secondary antibody
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Miss. Gwen Lagoda
Verified customer
提交于 Jul 29 2008