Application
Western blot
Loading amount
150000 cells
Gel Running Conditions
Reduced Denaturing (4-15%)
Sample
Simian Virus 40 (SV40) Cell lysate - nuclear (Sf9 insect cells)
Specification
Sf9 insect cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: milk
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Goat Anti-Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Bands
Specific: 40, 70 kDa
Exposure
2 minute(s) and 0 second(s)
Negative control
JCV VP1 positive cell lysate from Sf9 cells
GFP positive cell lysate from E.coli
Positive control
JCV VP2 positive cell lysate from Sf9 cells
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Nov 05 2013