Western blot abreview for Anti-STAT3 antibody [EPR787Y]

Excellent
Abreviews
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (4-15% TRIS-Glycine SDS PAGE)
Sample
Mouse Tissue lysate - whole (1) mouse embryonic spinal cord E11, 2) HEK293H)
Specification
1) mouse embryonic spinal cord E11, 2) HEK293H
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 5% Milk
Dilution
1/1000

Secondary antibody

Name
Non-Abcam antibody was used: goat-anti rabbit IgG-HRP coupled Jackson Immunores
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/20000

Detection

Detection method
ECL+
Bands
Specific: 90 kDa
Exposure
5 minute(s) and 0 second(s)

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提交于 Jul 28 2014

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