Application
Western blot
Sample
Mouse Cell lysate - whole cell (mES cells)
Loading amount
1 µg
Specification
mES cells
Gel Running Conditions
Non-reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1 X TBS + 0.1% Tween
Secondary antibody
Name
Non-Abcam antibody was used: Anti-mouse
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL+
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: ~80 kDa
Additional data
Additional Notes
Just loaded a cell pellet- probably about 0.1g of cells/lane.
Blocking in 5% milk
Primary antibody in 3% BSA, 1xTBS-T, + sodium azide.
I have reused the same aliquot 10+ times, over past 2 months.
Blocking in 5% milk
Primary antibody in 3% BSA, 1xTBS-T, + sodium azide.
I have reused the same aliquot 10+ times, over past 2 months.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jul 01 2010