Application
Western blot
Sample
Rat Cell lysate - other (neuron)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
15 µg
Treatment
different concentration of poisoning for 24hrs
Specification
neuron
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Milk
Dilution
1/1000
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: ????
Host species: Rabbit
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
Chemiluminescence
Exposure
20 second(s)
Bands
Specific: 62 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jan 24 2018