参阅全部 SOS2 一抗
经测试应用适用于: WB, IPmore details
预测可用于: Mouse, Rabbit, Horse, Guinea pig, Dog, Chimpanzee, Gorilla, Chinese hamster, Orangutan, Platypus, Elephant
Synthetic peptide, corresponding to a region within amino acids 1282 -1332 of Human SOS2 (NP_008870.2)
- HeLa and 293T whole cell lysate
存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
存储溶液Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
纯度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab85831 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 153 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
功能Promotes the exchange of Ras-bound GDP by GTP.
序列相似性Contains 1 DH (DBL-homology) domain.
Contains 1 N-terminal Ras-GEF domain.
Contains 1 PH domain.
Contains 1 Ras-GEF domain.
- Information by UniProt
- FLJ25596 antibody
- Guanine nucleotide exchange factor antibody
- Guanine nucleotide releasing factor antibody
All lanes : Anti-SOS2 antibody (ab85831) at 0.1 mg/ml
Lane 1 : Whole cell lysate from HeLa cells at 5 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 50 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Developed using the ECL technique.
Predicted band size: 153 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
Additional bands at: 150 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
Immunoprecipitation/ Western Blot of ab85831
Lane 1: ab85831 at 3µg/mg whole cell lysate. Lane 2: Control IgG.
ab85831 at 1µg/ml for WB. Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Chemiluminescence with an exposure time of 30 seconds.
ab85831 has not yet been referenced specifically in any publications.