Application
Western blot
Sample
Mouse Tissue lysate - whole (Muscle (lane 1) and spleen (lane 2))
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
45 µg
Specification
Muscle (lane 1) and spleen (lane 2)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Other product details
Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS Tween
Dilution
1/500
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Donkey Anti-Sheep/Goat IgG Antibody, HRP conjugate
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 35 kDa Non-specific: 17, 55, 110 kDa
Additional data
Additional Notes
Lane 1: mouse muscle (whole tissue)
Lane 2: mouse spleen (whole tissue)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Dec 22 2017