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Our Abpromise guarantee covers the use of ab9870 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 2 - 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 30-35 kDa.Can be blocked with Human SOCS1 peptide (ab23167).|
|ICC/IF||Use at an assay dependent concentration. PubMed: 21628332|
|Flow Cyt||Use at an assay dependent concentration. PubMed: 23298838
ab37373 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.
Flow cytometric analysis of HEK293 cells with ab9870 (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"