The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/5000. Detects a band of approximately 200 kDa (predicted molecular weight: 143 kDa).
Use at 2 µg/mg of lysate.
SLK is a serine/threonine protein kinase that is involved in cytoskeletal reorganization and apoptosis. SLK is a microtubule-associated protein inducing actin stress fiber disassembly, it also mediates apoptosis and it is ubiquitously expressed; highest expression is found in heart and in skeletal muscle. There are two named isoforms.
STE20 like serine/threonine-protein kinase antibody
STE20 related kinase antibody
Western blot - Anti-SLK antibody (ab70230)
All lanes : Anti-SLK antibody (ab70230) at 1 µg/ml
Lane 1 : MCF7 whole cell lysate at 50 µg Lane 2 : MCF7 whole cell lysate at 15 µg Lane 3 : MCF7 whole cell lysate at 5 µg Lane 4 : HeLa whole cell lysate at 15 µg Lane 5 : 293T whole cell lysate at 15 µg