Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Non-reduced Denaturing (10%)
Sample
Mouse Cell lysate - whole cell (primary hepatoctyes)
Specification
primary hepatoctyes
Blocking step
I-Block(Applied biosystems) as blocking agent for 30 minute(s) · Concentration: 2µg/mL · Temperature: 22°C
Other product details
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: I-Block buffer
Dilution
1/650
Secondary antibody
Secondary antibody
None used
Detection
Detection method
ECL
Bands
Specific: 37 kDa Non-specific: 30, 34, 43, 55, 80 kDa
Exposure
5 minute(s) and 0 second(s)
Additional data
Additional Notes
No specific band
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Oct 29 2013