SALL2 overexpression 293T裂解物(whole cell) (ab94229)
概述
-
产品名称
SALL2 overexpression 293T裂解物(whole cell) -
常规说明
ab94229 is a 293T cell transfected lysate in which Human SALL2 has been transiently over-expressed using a pCMV-SALL2 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
-
经测试应用
适用于: WBmore details
性能
-
Mycoplasma free
Yes -
形式
Liquid -
存放说明
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
存储溶液
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
研究领域
-
背景
The region-specific homeotic gene sal (spalt) of Drosophila encodes a zinc finger protein of unusual but characteristic structure. These unique features were used to isolate sal-like genes from humans. Two sal-like transcription units SALL1 and SALL2, located on chromosomes 16q12.1 and 14q11.1-q12.1 respectively, have been isolated and characterized. SALL1 and SALL2 transcripts are expressed in a limited number of adult organs, including the brain. SALL2 is evenly expressed in different brain areas. Transcripts of both genes can be detected in fetal brain neurons. The arrangement of sal-like zinc finger domains and their high degree of sequence similarity suggest a novel and conserved subfamily of human zinc finger transcription factors that is closely related to the Drosophila gene product encoded by the gene sal.
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab94229于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
Use at an assay dependent dilution.
|
说明 |
---|
WB
Use at an assay dependent dilution. |
图片
-
ab94229 at 15μg/lane on an SDS-PAGE gel
-
All lanes : Anti-SALL2 antibody (ab55723) at 1/500 dilution
Lane 1 :SALL2 overexpression 293T lysate (whole cell) (ab94229)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab94229 尚未被引用在任何文献中。