Anti-ARHGAP29抗体(ab85853)
Key features and details
- Rabbit polyclonal to ARHGAP29
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-ARHGAP29抗体 -
描述
兔多克隆抗体to ARHGAP29 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide corresponding to Human ARHGAP29 aa 1211-1261.
Database link: NP_004806.3 -
阳性对照
- HeLa, 293T and NIH3T3 whole cell lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab85853 was affinity purified using an epitope specific to ARHGAP29 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab85853于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 142 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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说明 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 142 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. Has strong activity toward RHOA, and weaker activity toward RAC1 and CDC42. May act as a specific effector of RAP2A to regulate Rho. In concert with RASIP1, suppresses RhoA signaling and dampens ROCK and MYH9 activities in endothelial cells and plays an essential role in blood vessel tubulogenesis. -
组织特异性
Widely expressed. Highly expressed in skeletal muscle and heart. Expressed at intermediate level in placenta, liver and pancreas. Weakly expressed in brain, lung and kidney. -
序列相似性
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 Rho-GAP domain. - Information by UniProt
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数据库链接
- Entrez Gene: 9411 Human
- Entrez Gene: 214137 Mouse
- Omim: 610496 Human
- SwissProt: Q52LW3 Human
- SwissProt: Q8CGF1 Mouse
- Unigene: 483238 Human
- Unigene: 229287 Mouse
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别名
- ARHGAP 29 antibody
- Arhgap29 antibody
- ARHGAP29 protein antibody
see all
图片
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All lanes : Anti-ARHGAP29 antibody (ab85853) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 142 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds -
Detection of ARHGAP29 by Western Blot of Immunprecipitate.
ab85853, at 1 µg/ml, staining ARHGAP29 in HeLa whole cell lysate immunoprecipitated using ab85853 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab85853 被引用在 2 文献中.
- Shimizu K et al. ARHGAP29 expression may be a novel prognostic factor of cell proliferation and invasion in prostate cancer. Oncol Rep 44:2735-2745 (2020). PubMed: 33125156
- Hernández CJ et al. PINK1 Silencing Modifies Dendritic Spine Dynamics of Mouse Hippocampal Neurons. J Mol Neurosci 69:570-579 (2019). PubMed: 31486971