Application
Western blot
Sample
Human Cell lysate - whole cell (H1299)
Loading amount
10 µg
Specification
H1299
Gel Running Conditions
Reduced Denaturing (7.5%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS-T
Secondary antibody
Name
Non-Abcam antibody was used: Donkey anti Rabbit IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL+
Exposure
1 second(s)
Bands
Specific: 50 kDa
Positive control
Flag Tagged Tip48
Additional data
Additional Notes
Lane 1,2,3: Endogenous Lane 4,5,6: Transfected Tip48 Flag tagged over-expression
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jun 16 2010