重组人MAP3K9蛋白(ab125554)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 85% Densitometry
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE
描述
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产品名称
重组人MAP3K9蛋白 -
生物活性
The specific activity of ab125554 was determined to be 116 nmol/min/mg.
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纯度
> 85 % Densitometry.
Purity was determined to be >85% by densitometry. -
表达系统
Baculovirus infected Sf9 cells -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Human -
预测分子量
77 kDa including tags -
氨基酸
1 to 433
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相关产品
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Substrate reagent
技术指标
Our Abpromise guarantee covers the use of ab125554 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Western blot
Functional Studies
SDS-PAGE
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形式
Liquid -
补充说明
ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- M3K9_HUMAN
- Map3k9
- MEKK9
see all -
功能
Activates the JUN N-terminal pathway. -
组织特异性
Expressed in epithelial tumor cell lines of colonic, breast and esophageal origin. -
序列相似性
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 1 protein kinase domain.
Contains 1 SH3 domain. -
翻译后修饰
Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-312 is likely to be the main autophosphorylation site. - Information by UniProt
图片
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab125554 尚未被引用在任何文献中。