重组人IRE1b蛋白(ab190994)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 95% SDS-PAGE
- Active: Yes
- Tags: proprietary tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
描述
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产品名称
重组人IRE1b蛋白 -
生物活性
The specific activity of ab190994 ws determined to be 2.6 nmol/ min/ mg.
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纯度
> 95 % SDS-PAGE.
Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
KQ AQPLDDPEAE QLTVVGKISF NPKDVLGRGA GGTFVFRGQF EGRAVAVKRL LRECFGLVRR EVQLLQESDR HPNVLRYFCT ERGPQFHYIA LELCRASLQE YVENPDLDRG GLEPEVVLQQ LMSGLAHLHS LHIVHRDLKP GNILITGPDS QGLGRVVLSD FGLCKKLPAG RCSFSLHSGI PGTEGWMAPE LLQLLPPDSP TSAVDIFSAG CVFYYVLSGG SHPFGDSLYR QANILTGAPC LAHLEEEVHD KVVARDLVGA MLSPLPQPRP SAPQVLAHPF FWSRAKQLQF FQDVSDWLEK ESEQEPLVRA LEAGGCAVVR DNWHEHISMP LQTDLRKFRS YKGTSVRDLL RAVRNKKHHY RELPVEVRQA LGQVPDGFVQ YFTNRFPRLL LHTHRAMRSC ASESLFLPYY PPDSEARRPC PGATGR -
预测分子量
80 kDa including tags -
氨基酸
499 to 926 -
标签
proprietary tag N-Terminus -
额外的序列信息
NM_033266.
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相关产品
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Substrate reagent
技术指标
Our Abpromise guarantee covers the use of ab190994 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Functional Studies
SDS-PAGE
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形式
Liquid -
补充说明
ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.50
Constituents: 25% Glycerol (glycerin, glycerine), 0.79% Tris HCl, 0.87% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.004% DTT, 0.002% PMSFThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- Endoplasmic reticulum to nucleus signaling 2
- Endoplasmic reticulum-to-nucleus signaling 2
- Endoribonuclease
see all -
功能
Induces translational repression through 28S ribosomal RNA cleavage in response to ER stress. Pro-apoptotic. Appears to play no role in the unfolded-protein response, unlike closely related proteins. -
序列相似性
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 1 KEN domain.
Contains 1 protein kinase domain. -
翻译后修饰
Autophosphorylated. -
细胞定位
Endoplasmic reticulum membrane. - Information by UniProt
图片
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The specific activity of IRE1b (ab190994) was determined to be 2.4 nmol/min/mg as per activity assay protocol and was equivalent to 2.3 nmol/min/mg as per radiometric assay
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SDS PAGE analysis of ab190994
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SDS PAGE analysis of ab190994
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The specific activity of ab190994 ws determined to be 2.6 nmol/ min/ mg.
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The purity of ab190994 was determined to be >95% by densitometry, approx MW ~80 kDa.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab190994 尚未被引用在任何文献中。